1m4x
From Proteopedia
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PBCV-1 virus capsid, quasi-atomic model
Overview
Paramecium bursaria Chlorella virus type 1 (PBCV-1) is a very large, icosahedral virus containing an internal membrane enclosed within a, glycoprotein coat consisting of pseudohexagonal arrays of trimeric, capsomers. Each capsomer is composed of three molecules of the major, capsid protein, Vp54, the 2.0-A resolution structure of which is reported, here. Four N-linked and two O-linked glycosylation sites were identified., The N-linked sites are associated with nonstandard amino acid motifs as a, result of glycosylation by virus-encoded enzymes. Each monomer of the, trimeric structure consists of two eight-stranded, antiparallel, beta-barrel, "jelly-roll" domains related by a pseudo-sixfold rotation., The fold of the monomer and the pseudo-sixfold symmetry of the capsomer, resembles that of the major coat proteins in the double-stranded DNA, bacteriophage PRD1 and the double-stranded DNA human adenoviruses, as well, as the viral proteins VP2-VP3 of picornaviruses. The structural, similarities among these diverse groups of viruses, whose hosts include, bacteria, unicellular eukaryotes, plants, and mammals, make it probable, that their capsid proteins have evolved from a common ancestor that had, already acquired a pseudo-sixfold organization. The trimeric capsid, protein structure was used to produce a quasi-atomic model of the 1,900-A, diameter PBCV-1 outer shell, based on fitting of the Vp54 crystal, structure into a three-dimensional cryoelectron microscopy image, reconstruction of the virus.
About this Structure
1M4X is a Single protein structure of sequence from Paramecium bursaria chlorella virus type 1 (pbcv-1). Full crystallographic information is available from OCA.
Reference
The structure and evolution of the major capsid protein of a large, lipid-containing DNA virus., Nandhagopal N, Simpson AA, Gurnon JR, Yan X, Baker TS, Graves MV, Van Etten JL, Rossmann MG, Proc Natl Acad Sci U S A. 2002 Nov 12;99(23):14758-63. Epub 2002 Oct 31. PMID:12411581
Page seeded by OCA on Tue Nov 20 21:08:57 2007
