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1mbm
From Proteopedia
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NSP4 proteinase from Equine Arteritis Virus
Overview
Arteriviruses are enveloped, positive-stranded RNA viruses and include, pathogens of major economic concern to the swine- and horse-breeding, industries. The arterivirus replicase gene encodes two large precursor, polyproteins that are processed by the viral main proteinase nonstructural, protein 4 (nsp4). The three-dimensional structure of the 21-kDa nsp4 from, the arterivirus prototype equine arteritis virus has been determined to, 2.0 A resolution. Nsp4 adopts the smallest known chymotrypsin-like fold, with a canonical catalytic triad of Ser-120, His-39, and Asp-65, as well, as a novel alpha/beta C-terminal extension domain that may play a role in, mediating protein-protein interactions. In different copies of nsp4 in the, asymmetric unit, the oxyanion hole adopts either a collapsed inactive, conformation or the standard active conformation, which may be a novel way, of regulating proteolytic activity.
About this Structure
1MBM is a Single protein structure of sequence from Equine arteritis virus. Full crystallographic information is available from OCA.
Reference
Structure of arterivirus nsp4. The smallest chymotrypsin-like proteinase with an alpha/beta C-terminal extension and alternate conformations of the oxyanion hole., Barrette-Ng IH, Ng KK, Mark BL, Van Aken D, Cherney MM, Garen C, Kolodenko Y, Gorbalenya AE, Snijder EJ, James MN, J Biol Chem. 2002 Oct 18;277(42):39960-6. Epub 2002 Aug 5. PMID:12163505
Page seeded by OCA on Tue Nov 20 21:18:42 2007
