1mey
From Proteopedia
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CRYSTAL STRUCTURE OF A DESIGNED ZINC FINGER PROTEIN BOUND TO DNA
Overview
Considerable recent effort has been devoted to the design and selection of, sequence-specific DNA binding proteins based on tandem arrays of Cys2His2, zinc finger domains. While the DNA binding properties of these designed, proteins have been studied extensively, the structural basis for, site-specific binding has not been examined experimentally. Here we report, the crystal structure of a complex between a protein comprised of three, consensus-sequence-based zinc finger domains and an oligonucleotide, corresponding to a favourable DNA binding site. This structure reveals, relatively simple modular interactions and structural adaptations that, compensate for differences in contact residue side-chain lengths.
About this Structure
1MEY is a Protein complex structure of sequences from [1] with ZN and CL as ligands. Full crystallographic information is available from OCA.
Reference
A 2.2 A resolution crystal structure of a designed zinc finger protein bound to DNA., Kim CA, Berg JM, Nat Struct Biol. 1996 Nov;3(11):940-5. PMID:8901872 [[Category: ]]
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