1mfe

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RECOGNITION OF A CELL-SURFACE OLIGO-SACCHARIDE OF PATHOGENIC SALMONELLA BY AN ANTIBODY FAB FRAGMENT

Overview

The 2.05 angstrom (A) resolution crystal structure of a, dodecasaccharide-Fab complex revealed an unusual carbohydrate recognition, site, defined by aromatic amino acids and a structured water molecule, rather than the carboxylic acid and amide side chains and a structured, water molecule, rather than the carboxylic acid and amide side chains that, are features of transport and other carbohydrate binding proteins. A, trisaccharide epitope of a branched bacterial lipopolysaccharide fills, this hydrophobic pocket (8 A deep by 7 A wide) in an entropy-assisted, association (association constant = 2.05 x 10(5) liters per mole, enthalpy, = -20.5 +/- 1.7 kilojoules per mole, and temperature times entropy = +10.0, +/- 2.9 kilojoules per mole). The requirement for the complementarity of, van der Waals surfaces and the requirements of saccharide-saccharide and, protein-saccharide hydrogen-bonding networks determine the antigen, conformation adopted in the bound state.

About this Structure

1MFE is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.

Reference

Recognition of a cell-surface oligosaccharide of pathogenic Salmonella by an antibody Fab fragment., Cygler M, Rose DR, Bundle DR, Science. 1991 Jul 26;253(5018):442-5. PMID:1713710

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