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1mis
From Proteopedia
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STRUCTURE OF RNA (5'-R(GP*CP*GP*GP*AP*CP*GP*C)-3') ANTI-PARALLEL RNA DUPLEX WITH TANDEM G:A MISMATCHES, NMR, MINIMIZED AVERAGE STRUCTURE
Overview
The three-dimensional solution structure of the RNA self-complementary, duplex [sequence: see text] was derived from two-dimensional NMR and the, iterative relaxation matrix approach. Each GA mismatch forms two hydrogen, bonds: A-NH6 to G-O6 and A-N1 to G-NH1 (imino). This is the first, three-dimensional RNA structure with imino hydrogen-bonded tandem GA, mismatches. This GA structure is totally different from the sheared tandem, GA structure in [sequence: see text] which also has two hydrogen bonds:, A-N7 to G-NH2 and A-NH6 to G-N3 [SantaLucia, J., Jr., & Turner, D. H., (1993) Biochemistry 32, 12612-12623]. In particular, the sheared and imino, GA mismatches produce a narrowing and widening of the backbone, respectively. The results show that substitutions of Watson-Crick base, pairs can have dramatic effects on the three-dimensional structures of, adjacent non-Watson-Crick paired regions; i.e., the structure depends on, sequence context. Thus compensating substitutions in site-directed, mutagenesis experiments may not always restore biological activities.
About this Structure
1MIS is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
Solution structure of (rGCGGACGC)2 by two-dimensional NMR and the iterative relaxation matrix approach., Wu M, Turner DH, Biochemistry. 1996 Jul 30;35(30):9677-89. PMID:8703939
Page seeded by OCA on Sun Nov 25 04:14:46 2007
