1mve
From Proteopedia
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Crystal structure of a natural circularly-permutated jellyroll protein: 1,3-1,4-beta-D-glucanase from Fibrobacter succinogenes
Overview
The 1,3-1,4-beta-D-glucanase from Fibrobacter succinogenes, (Fsbeta-glucanase) is classified as one of the family 16 glycosyl, hydrolases. It hydrolyzes the glycosidic bond in the mixed-linked glucans, containing beta-1,3- and beta-1,4-glycosidic linkages. We constructed a, truncated form of recombinant Fsbeta-glucanase containing the catalytic, domain from amino acid residues 1-258, which exhibited a higher thermal, stability and enzymatic activity than the full-length enzyme. The crystal, structure of the truncated Fsbeta-glucanase was solved at a resolution of, 1.7A by the multiple wavelength anomalous dispersion (MAD) method using, the anomalous signals from the seleno-methionine-labeled protein. The, overall topology of the truncated Fsbeta-glucanase consists mainly of two, eight-stranded anti-parallel beta-sheets arranged in a jellyroll, beta-sandwich, similar to the fold of many glycosyl hydrolases and, carbohydrate-binding modules. Sequence comparison with other bacterial, glucanases showed that Fsbeta-glucanase is the only naturally occurring, circularly permuted beta-glucanase with reversed sequences. Structural, comparison shows that the engineered circular-permuted Bacillus enzymes, are more similar to their parent enzymes with which they share, approximately 70% sequence identity, than to the naturally occurring, Fsbeta-glucanase of similar topology with 30% identity. This result, suggests that protein structure relies more on sequence identity than, topology. The high-resolution structure of Fsbeta-glucanase provides a, structural rationale for the different activities obtained from a series, of mutant glucanases and a basis for the development of engineered enzymes, with increased activity and structural stability.
About this Structure
1MVE is a Single protein structure of sequence from Fibrobacter succinogenes with CA as ligand. Active as Licheninase, with EC number 3.2.1.73 Full crystallographic information is available from OCA.
Reference
Crystal structure of a natural circularly permuted jellyroll protein: 1,3-1,4-beta-D-glucanase from Fibrobacter succinogenes., Tsai LC, Shyur LF, Lee SH, Lin SS, Yuan HS, J Mol Biol. 2003 Jul 11;330(3):607-20. PMID:12842475
Page seeded by OCA on Tue Nov 20 21:43:45 2007
