1mw9
From Proteopedia
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Crystal Structure of H365R mutant of 67 kDA N-terminal fragment of E. coli DNA Topoisomerase I
Overview
In order to gain insights into the mechaism of ssDNA binding and, recognition by Escherichia coli DNA topoisomerase I, the structure of the, 67 kDa N-terminal fragment of topoisomerase I was solved in complex with, ssDNA. The structure reveals a new conformational stage in the multistep, catalytic cycle of type IA topoisomerases. In the structure, the ssDNA, binding groove leading to the active site is occupied, but the active site, is not fully formed. Large conformational changes are not seen; instead, a, single helix parallel to the ssDNA binding groove shifts to clamp the, ssDNA. The structure helps clarify the temporal sequence of conformational, events, starting from an initial empty enzyme and proceeding to a, ssDNA-occupied and catalytically competent active site.
About this Structure
1MW9 is a Single protein structure of sequence from Escherichia coli with SO4 as ligand. Active as DNA topoisomerase, with EC number 5.99.1.2 Full crystallographic information is available from OCA.
Reference
Structure of a complex between E. coli DNA topoisomerase I and single-stranded DNA., Perry K, Mondragon A, Structure. 2003 Nov;11(11):1349-58. PMID:14604525
Page seeded by OCA on Tue Nov 20 21:45:26 2007
