1n27

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Solution structure of the PWWP domain of mouse Hepatoma-derived growth factor, related protein 3

Overview

Among the many PWWP-containing proteins, the largest group of homologous, proteins is related to hepatoma-derived growth factor (HDGF). Within a, well-conserved region at the extreme N-terminus, HDGF and five, HDGF-related proteins (HRPs) always have a PWWP domain, which is a module, found in many chromatin-associated proteins. In this study, we determined, the solution structure of the PWWP domain of HDGF-related protein-3, (HRP-3) by NMR spectroscopy. The structure consists of a five-stranded, beta-barrel with a PWWP-specific long loop connecting beta2 and beta3, (PR-loop), followed by a helical region including two alpha-helices. Its, structure was found to have a characteristic solvent-exposed hydrophobic, cavity, which is composed of an abundance of aromatic residues in the, beta1/beta2 loop (beta-beta arch) and the beta3/beta4 loop. A similar, ligand binding cavity occurs at the corresponding position in the Tudor, chromo, and MBT domains, which have structural and probable evolutionary, relationships with PWWP domains. These findings suggest that the PWWP, domains of the HDGF family bind to some component of chromatin via the, cavity.

About this Structure

1N27 is a Single protein structure of sequence from Mus musculus. Full crystallographic information is available from OCA.

Reference

Solution structure of the PWWP domain of the hepatoma-derived growth factor family., Nameki N, Tochio N, Koshiba S, Inoue M, Yabuki T, Aoki M, Seki E, Matsuda T, Fujikura Y, Saito M, Ikari M, Watanabe M, Terada T, Shirouzu M, Yoshida M, Hirota H, Tanaka A, Hayashizaki Y, Guntert P, Kigawa T, Yokoyama S, Protein Sci. 2005 Mar;14(3):756-64. Epub 2005 Feb 2. PMID:15689505

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