1n4l
From Proteopedia
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A DNA analogue of the polypurine tract of HIV-1
Overview
The polypurine tract (PPT) from the HIV-1 genome is resistant to digestion, by reverse transcriptase following (-)-strand synthesis and is used to, prime (+)-strand synthesis during retroviral replication. We have, determined the crystal structure of the asymmetric DNA/DNA analog16-mer, duplex (CTTTTTAAAAGAAAAG/CTTTTCTTTTAAAAAG) comprising most of the, "visible" portion of the RNA:DNA hybrid from the polypurine tract of, HIV-1, which was previously reported in a complex with HIV-1 reverse, transcriptase. Our 16-mer completely encompasses a 10-mer DNA duplex, analog of the HIV-1 PPT. We report here a detailed analysis of our B' form, 16-mer DNA structure, including three full pure A-tracts, as well as a, comparative structural analysis with polypurine tract and other, A-tract-containing nucleic acid structures. Our analysis reveals that the, polypurine tract structures share structural features despite being, different nucleic acid forms (i.e. DNA:DNA versus RNA:DNA). In addition, the previously reported A-tract-containing DNA molecules bound to, topoisomerase I are remarkably similar to our polypurine tract 16-mer, structure. On the basis of our analysis, we suggest that the specific, topology of long pure A-tracts is remarkably comparable across a wide, array of biological environments.
About this Structure
1N4L is a Single protein structure of sequence from Moloney murine leukemia virus. Active as RNA-directed DNA polymerase, with EC number 2.7.7.49 Full crystallographic information is available from OCA.
Reference
Staying straight with A-tracts: a DNA analog of the HIV-1 polypurine tract., Cote ML, Pflomm M, Georgiadis MM, J Mol Biol. 2003 Jun 27;330(1):57-74. PMID:12818202
Page seeded by OCA on Thu Nov 8 14:21:14 2007
