1n71
From Proteopedia
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Crystal structure of aminoglycoside 6'-acetyltransferase type Ii in complex with coenzyme A
Overview
The rise of antibiotic resistance as a public health concern has led to, increased interest in studying the ways in which bacteria avoid the, effects of antibiotics. Enzymatic inactivation by several families of, enzymes has been observed to be the predominant mechanism of resistance to, aminoglycoside antibiotics such as kanamycin and gentamicin. Despite the, importance of acetyltransferases in bacterial resistance to aminoglycoside, antibiotics, relatively little is known about their structure and, mechanism. Here we report the three-dimensional atomic structure of the, aminoglycoside acetyltransferase AAC(6')-Ii in complex with coenzyme A, (CoA). This structure unambiguously identifies the physiologically, relevant AAC(6')-Ii dimer species, and reveals that the enzyme structure, is similar in the AcCoA and CoA bound forms. AAC(6')-Ii is a member of the, GCN5-related N-acetyltransferase (GNAT) superfamily of acetyltransferases, a diverse group of enzymes that possess a conserved structural motif, despite low sequence homology. AAC(6')-Ii is also a member of a subset of, enzymes in the GNAT superfamily that form multimeric complexes. The dimer, arrangements within the multimeric GNAT superfamily members are compared, revealing that AAC(6')-Ii forms a dimer assembly that is different from, that observed in the other multimeric GNAT superfamily members. This, different assembly may provide insight into the evolutionary processes, governing dimer formation.
About this Structure
1N71 is a Single protein structure of sequence from Enterococcus faecium with SO4 and COA as ligands. Full crystallographic information is available from OCA.
Reference
X-ray structure of the AAC(6')-Ii antibiotic resistance enzyme at 1.8 A resolution; examination of oligomeric arrangements in GNAT superfamily members., Burk DL, Ghuman N, Wybenga-Groot LE, Berghuis AM, Protein Sci. 2003 Mar;12(3):426-37. PMID:12592013
Page seeded by OCA on Sat Nov 24 22:39:49 2007
