1nbk
From Proteopedia
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The structure of RNA aptamer for HIV Tat complexed with two argininamide molecules
Overview
An RNA aptamer containing two binding sites exhibits extremely high, affinity to the HIV Tat protein. We have determined the structure of the, aptamer complexed with two argininamide molecules. Two adjacent U:A:U base, triples were formed, which widens the major groove to make space for the, two argininamide molecules. The argininamide molecules bind to the G bases, through hydrogen bonds. The binding is stabilized through stacking, interactions. The structure of the aptamer complexed with a Tat-derived, arginine-rich peptide was also characterized. It was suggested that the, aptamer structure is similar for both complexes and that the aptamer, interacts with two different arginine residues of the peptide, simultaneously at the two binding sites, which could explain the high, affinity to Tat.
About this Structure
1NBK is a Protein complex structure of sequences from [1] with GND as ligand. Full crystallographic information is available from OCA.
Reference
Structural basis of the highly efficient trapping of the HIV Tat protein by an RNA aptamer., Matsugami A, Kobayashi S, Ouhashi K, Uesugi S, Yamamoto R, Taira K, Nishikawa S, Kumar PK, Katahira M, Structure. 2003 May;11(5):533-45. PMID:12737819
Page seeded by OCA on Thu Nov 8 14:21:43 2007
