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1a4w

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Revision as of 09:40, 30 October 2007 by OCA (Talk | contribs)
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1a4w, resolution 1.8Å

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CRYSTAL STRUCTURES OF THROMBIN WITH THIAZOLE-CONTAINING INHIBITORS: PROBES OF THE S1' BINDING SITE

Overview

Structures of the blood clotting enzyme thrombin complexed with hirugen, and two active site inhibitors, RWJ-50353, 10080(N-methyl-D-phenylalanyl-N-[5-[(aminoiminomethyl)amino]-1-, [[(2-benzothiazolyl)carbonyl]butyl]-L-prolinamide trifluoroacetate, hydrate) and RWJ-50215 (N-[4-(aminoiminomethyl)amino-1-[2-, (thiazol-2-ylcarbonylethyl)piperidin-, 1-ylcarbonyl]butyl]-5-(dimethylamino)naphthalenesulfonamide, trifluoroacetate hydrate), were determined by x-ray crystallography. The, refinements converged at R values of 0.158 in the 7.0-2.3-A range for, RWJ-50353 and 0.155 in the 7.0-1.8-A range for RWJ-50215. Interactions, between the protein and the thiazole rings of the two inhibitors provide, new valuable information about the S1' binding site of thrombin. The, RWJ-50353 inhibitor consists ... [(full description)]

About this Structure

1A4W is a [Protein complex] structure of sequences from [Homo sapiens] with NA, ANS and KTH as [ligands]. Active as [Thrombin], with EC number [3.4.21.5]. Structure known Active Site: CAT. Full crystallographic information is available from [OCA].

Reference

Crystal structures of thrombin with thiazole-containing inhibitors: probes of the S1' binding site., Matthews JH, Krishnan R, Costanzo MJ, Maryanoff BE, Tulinsky A, Biophys J. 1996 Nov;71(5):2830-9. PMID:8913620

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