1nlq

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1nlq, resolution 1.50Å

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The crystal structure of Drosophila NLP-core provides insight into pentamer formation and histone binding

Overview

The nucleoplasmin-like protein from Drosophila (dNLP) functions as a, chaperone for core histones and may remodel chromatin in embryos. We now, report the crystal structure of a dNLP-core pentamer at 1.5 A resolution., The monomer has an eight-stranded, beta barrel topology that is similar to, nucleoplasmin (Np). However, a signature beta hairpin is tucked in along, the lateral surface of the dNLP-core pentamer, while it extends outward in, the Np-core decamer. Drosophila NLP and Np both assemble histone octamers., This process may require each chaperone to form a decamer, which would, create symmetric binding sites for the histones. Conformational, differences between dNLP and Np may reflect their different oligomeric, states, while a conserved, nonpolar subunit interface may allow, conformational plasticity during histone binding.

About this Structure

1NLQ is a Single protein structure of sequence from Drosophila melanogaster with MG as ligand. Full crystallographic information is available from OCA.

Reference

The crystal structure of Drosophila NLP-core provides insight into pentamer formation and histone binding., Namboodiri VM, Dutta S, Akey IV, Head JF, Akey CW, Structure. 2003 Feb;11(2):175-86. PMID:12575937

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