1nr4
From Proteopedia
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High resolution crystal structures of thymus and activation-regulated chemokine
Overview
Thymus and activation-regulated chemokine (TARC) is a CC chemokine that is, mainly expressed in the thymus. TARC interacts primarily with the CCR4, receptor and to a lesser extent with the CCR8 receptor. The structures of, TARC have been solved by molecular replacement in two space groups, triclinic (P1) and tetragonal (P4(1)), and refined to resolutions of 1.72, and 2.1 A, respectively, with R factors of 19.8% (R(free) = 24.1%) and, 19.8% (R(free) = 27.7%), respectively. The search model originated from, the crystal structure of another chemokine, RANTES, and proved to be only, modestly similar to the refined structure of TARC. Whereas the tetragonal, structure was easily solved using the program AMoRe, solution of the, triclinic structure proved to be quite challenging and was obtained by, combining the results from four different molecular-replacement programs, (AMoRe, CNS, BEAST and EPMR), with subsequent extension of the gathered, information. The tertiary structure of TARC is similar to that of other CC, chemokines, with a three-stranded antiparallel beta-sheet flanked by a, C-terminal helix. Both quaternary structures consist of dimers, which in, the triclinic crystals pack further into tetramers. The TARC dimers are, similar to those observed previously in the crystal structures of both, MCP-1 and RANTES.
About this Structure
1NR4 is a Single protein structure of sequence from [1] with SO4 as ligand. Full crystallographic information is available from OCA.
Reference
Structures of thymus and activation-regulated chemokine (TARC)., Asojo OA, Boulegue C, Hoover DM, Lu W, Lubkowski J, Acta Crystallogr D Biol Crystallogr. 2003 Jul;59(Pt 7):1165-73. Epub 2003, Jun 27. PMID:12832759
Page seeded by OCA on Mon Nov 12 18:24:18 2007
Categories: Single protein | Asojo, O.A. | Boulegue, C. | Hoover, D.M. | Lu, W. | Lubkowski, J. | SO4 | Cc-chemokine | Chemokine | Chemotaxis | Cytokine | Tarc
