1nqz
From Proteopedia
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The structure of a CoA pyrophosphatase from D. Radiodurans complexed with a magnesium ion
Overview
Gene Dr1184 from Deinococcus radiodurans codes for a Nudix enzyme, (DR-CoAse) that hydrolyzes the pyrophosphate moiety of coenzyme A (CoA)., Nudix enzymes with the same specificity have been found in yeast, humans, and mice. The three-dimensional structure of DR-CoAse, the first of a, Nudix hydrolase with this specificity, reveals that this enzyme contains, in addition to the fold observed in other Nudix enzymes, insertions that, are characteristic of a CoA-hydrolyzing Nudix subfamily. The structure of, the complex of the enzyme with Mg(2+), its activating cation, reveals the, position of the catalytic site. A helix, part of the N-terminal insertion, partially occludes the binding site and has to change its position to, permit substrate binding. Comparison of the structure of DR-CoAse to those, of other Nudix enzymes, together with the location in the structure of the, sequence characteristic of CoAses, suggests a mode of binding of the, substrate to the enzyme that is compatible with all available data.
About this Structure
1NQZ is a Single protein structure of sequence from Deinococcus radiodurans with MG as ligand. Active as Nucleotide diphosphatase, with EC number 3.6.1.9 Full crystallographic information is available from OCA.
Reference
Structure of a coenzyme A pyrophosphatase from Deinococcus radiodurans: a member of the Nudix family., Kang LW, Gabelli SB, Bianchet MA, Xu WL, Bessman MJ, Amzel LM, J Bacteriol. 2003 Jul;185(14):4110-8. PMID:12837785
Page seeded by OCA on Sat Nov 24 23:36:50 2007
