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1o7d
From Proteopedia
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THE STRUCTURE OF THE BOVINE LYSOSOMAL A-MANNOSIDASE SUGGESTS A NOVEL MECHANISM FOR LOW PH ACTIVATION
Overview
Lysosomal alpha-mannosidase (LAM: EC 3.2.1.24) belongs to the, sequence-based glycoside hydrolase family 38 (GH38). Two other mammalian, GH38 members, Golgi alpha-mannosidase II (GIIAM) and cytosolic, alpha-mannosidase, are expressed in all tissues. In humans, cattle, cat, and guinea pig, lack of lysosomal alpha-mannosidase activity causes the, autosomal recessive disease alpha-mannosidosis. Here, we describe the, three-dimensional structure of bovine lysosomal alpha-mannosidase (bLAM), at 2.7A resolution and confirm the solution state dimer by electron, microscopy. We present the first structure of a mammalian GH38 enzyme that, offers indications for the signal areas for mannose phosphorylation, suggests a previously undetected mechanism of low-pH activation and, provides a template for further biochemical studies of the family 38, glycoside hydrolases as well as lysosomal transport. Furthermore, it, provides a basis for understanding the human form of alpha-mannosidosis at, the atomic level. The atomic coordinates and structure factors have been, deposited in the Protein Data Bank (accession codes 1o7d and r1o7dsf).
About this Structure
1O7D is a Single protein structure of sequence from Bos taurus with , , and as ligands. Active as Alpha-mannosidase, with EC number 3.2.1.24 Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
The structure of bovine lysosomal alpha-mannosidase suggests a novel mechanism for low-pH activation., Heikinheimo P, Helland R, Leiros HK, Leiros I, Karlsen S, Evjen G, Ravelli R, Schoehn G, Ruigrok R, Tollersrud OK, McSweeney S, Hough E, J Mol Biol. 2003 Mar 28;327(3):631-44. PMID:12634058
Page seeded by OCA on Sun Feb 3 09:54:25 2008
Categories: Alpha-mannosidase | Bos taurus | Single protein | Evjen, G. | Heikinheimo, P. | Helland, R. | Hough, E. | Karlsen, S. | Leiros, H.S. | Leiros, I. | Mcsweeney, S. | Ravelli, R. | Ruigrok, R. | Schoehn, G. | Tollersrud, O.K. | NAG | SO4 | TRS | ZN | A-mannosidase | Glycosyl hydrolase family 38 | Lysosomal
