1ob1

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spinqualitylabelsall models 1ob1, resolution 2.90Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

CRYSTAL STRUCTURE OF A FAB COMPLEX WHITH PLASMODIUM FALCIPARUM MSP1-19

Overview

Merozoite surface protein 1 (MSP1) is the major protein component on the, surface of the merozoite, the erythrocyte-invasive form of the malaria, parasite Plasmodium. Present in all species of Plasmodium, it undergoes, two distinct proteolytic maturation steps during the course of merozoite, development that are essential for invasion of the erythrocyte. Antibodies, specific for the C-terminal maturation product, MSP1-19, can inhibit, erythrocyte invasion and parasite growth. This polypeptide is therefore, considered to be one of the more promising malaria vaccine candidates. We, describe here the crystal structure of recombinant MSP1-19 from, P.falciparum (PfMSP1-19), the most virulent species of the parasite in, humans, as a complex with the Fab fragment of the monoclonal antibody, G17.12. This antibody recognises a discontinuous epitope comprising 13, residues on the first epidermal growth factor (EGF)-like domain of, PfMSP1-19. Although G17.12 was raised against the recombinant antigen, expressed in an insect cell/baculovirus system, it binds uniformly to the, surface of merozoites from the late schizont stage, showing that the, cognate epitope is exposed on the naturally occurring MSP1 polypeptide, complex. Although the epitope includes residues that have been mapped to, regions recognised by invasion-inhibiting antibodies studied by other, workers, G17.12 does not inhibit erythrocyte invasion or MSP1 processing.

About this Structure

1OB1 is a Single protein structure of sequence from Mus musculus and Plasmodium falciparum with as ligand. Full crystallographic information is available from OCA.

Reference

Crystal structure of a Fab complex formed with PfMSP1-19, the C-terminal fragment of merozoite surface protein 1 from Plasmodium falciparum: a malaria vaccine candidate., Pizarro JC, Chitarra V, Verger D, Holm I, Petres S, Dartevelle S, Nato F, Longacre S, Bentley GA, J Mol Biol. 2003 May 16;328(5):1091-103. PMID:12729744

Page seeded by OCA on Fri Feb 15 16:33:42 2008