1osp
From Proteopedia
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CRYSTAL STRUCTURE OF OUTER SURFACE PROTEIN A OF BORRELIA BURGDORFERI COMPLEXED WITH A MURINE MONOCLONAL ANTIBODY FAB
Overview
OspA (outer surface protein A) is an abundant immunogenic lipoprotein of, the Lyme disease spirochete Borrelia burgdorferi. The crystal structure of, a soluble recombinant form of OspA was solved in a complex with the Fab, fragment of mouse monoclonal antibody 184.1 and refined to a resolution of, 1.9 A. OspA has a repetitive antiparallel beta topology with an unusual, nonglobular region of "freestanding" sheet connecting globular N- and, C-terminal domains. Arrays of residues with alternating charges are a, predominant feature of the folding pattern in the nonglobular region. The, 184.1 epitope overlaps with a well conserved surface in the N-terminal, domain, and a hydrophobic cavity buried in a positively charged cleft in, the C-terminal domain is a potential binding site for an unknown ligand., An exposed variable region on the C-terminal domain of OspA is predicted, to be an important factor in the worldwide effectiveness of OspA-based, vaccines.
About this Structure
1OSP is a Protein complex structure of sequences from Borrelia burgdorferi and Mus musculus. Full crystallographic information is available from OCA.
Reference
Crystal structure of Lyme disease antigen outer surface protein A complexed with an Fab., Li H, Dunn JJ, Luft BJ, Lawson CL, Proc Natl Acad Sci U S A. 1997 Apr 15;94(8):3584-9. PMID:9108020
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