1ou8
From Proteopedia
|
structure of an AAA+ protease delivery protein in complex with a peptide degradation tag
Overview
Substrate selection by AAA+ ATPases that function to unfold proteins or, alter protein conformation is often regulated by delivery or adaptor, proteins. SspB is a protein dimer that binds to the ssrA degradation tag, and delivers proteins bearing this tag to ClpXP, an AAA+ protease, for, degradation. Here, we describe the structure of the peptide binding domain, of H. influenzae SspB in complex with an ssrA peptide at 1.6 A resolution., The ssrA peptides are bound in well-defined clefts located at the extreme, ends of the SspB homodimer. SspB contacts residues within the N-terminal, and central regions of the 11 residue ssrA tag but leaves the C-terminal, residues exposed and positioned to dock with ClpX. This structure, taken, together with biochemical analysis of SspB, suggests mechanisms by which, proteins like SspB escort substrates to AAA+ ATPases and enhance the, specificity and affinity of target recognition.
About this Structure
1OU8 is a Single protein structure of sequence from Haemophilus influenzae with MG as ligand. Full crystallographic information is available from OCA.
Reference
Structure of a delivery protein for an AAA+ protease in complex with a peptide degradation tag., Levchenko I, Grant RA, Wah DA, Sauer RT, Baker TA, Mol Cell. 2003 Aug;12(2):365-72. PMID:14536076
Page seeded by OCA on Tue Nov 20 23:12:30 2007
