1oxc
From Proteopedia
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LecB (PA-LII) in complex with FUCOSE
Overview
The crystal structure of Pseudomonas aeruginosa fucose-specific lectin, LecB was determined in its metal-bound and metal-free state as well as in, complex with fucose, mannose and fructopyranose. All three monosaccharides, bind isosterically via direct interactions with two calcium ions as well, as direct hydrogen bonds with several side-chains. The higher affinity for, fucose is explained by the details of the binding site around C6 and O1 of, fucose. In the mannose and fructose complexes, a carboxylate oxygen atom, and one or two hydroxyl groups are partly shielded from solvent upon sugar, binding, preventing them from completely fulfilling their hydrogen bonding, potential. In the fucose complex, no such defects are observed. Instead, C6 makes favourable interactions with a small hydrophobic patch. Upon, demetallization, the C terminus as well as the otherwise rigid, metal-binding loop become more mobile and adopt multiple conformations.
About this Structure
1OXC is a Single protein structure of sequence from Pseudomonas aeruginosa with FUL, FUC, CA and SO4 as ligands. Full crystallographic information is available from OCA.
Reference
Structural basis of carbohydrate recognition by the lectin LecB from Pseudomonas aeruginosa., Loris R, Tielker D, Jaeger KE, Wyns L, J Mol Biol. 2003 Aug 22;331(4):861-70. PMID:12909014
Page seeded by OCA on Sun Nov 25 01:36:08 2007
Categories: Pseudomonas aeruginosa | Single protein | Jaeger, K.E. | Loris, R. | Tielker, D. | Wyns, L. | CA | FUC | FUL | SO4 | Carbohydrate | Lectin
