1p13
From Proteopedia
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Crystal Structure of the Src SH2 Domain Complexed with Peptide (SDpYANFK)
Overview
The receptor protein tyrosine phosphatase alpha (RPTPalpha) is a, transmembrane receptor with two intracellular protein tyrosine phosphatase, domains, a catalytically active membrane proximal domain (D1) and a, membrane distal phosphatase domain with minimal catalytic activity (D2)., Here we elucidate the crystal structure of RPTPalpha's D2 domain. Unlike, D1, D2 exists as a monomer and lacks the N-terminal inhibitory wedge, motif. The N-terminal portion of D2 is disordered, and this region linking, D1 to D2 is proteolytically labile in solution whether part of D2 alone or, tethered to D1, indicating that the polypeptide backbone of this part of, D2 is highly flexible, and therefore accessible to proteases under native, conditions. Furthermore, we have crystallized the SH2 domain of the, protein tyrosine kinase c-Src, a RPTPalpha substrate, with a, phosphopeptide encompassing the C-terminal phosphorylation site of D2, (pTyr789). The SH2 domain of Src binds RPTPalpha in an extended, conformation. The structural and functional data support a D1-D2, arrangement with significant flexibility between phosphatase domains of, RPTPalpha that is likely to be important for dynamic alterations in intra-, and/or intermolecular interactions that are critical for RPTPalpha, function.
About this Structure
1P13 is a Single protein structure of sequence from Gallus gallus with CAC as ligand. Active as Transferase, with EC number and 2.7.10.2 2.7.10.1 and 2.7.10.2 Full crystallographic information is available from OCA.
Reference
The structure of the membrane distal phosphatase domain of RPTPalpha reveals interdomain flexibility and an SH2 domain interaction region., Sonnenburg ED, Bilwes A, Hunter T, Noel JP, Biochemistry. 2003 Jul 8;42(26):7904-14. PMID:12834342
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