1p79

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spinqualitylabelsall models 1p79, resolution 1.10Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

Crystal structure of a bulged RNA tetraplex: implications for a novel binding site in RNA tetraplex

Overview

Bulges are an important structural motif in RNA and can be used as, recognition and interaction sites in RNA-protein interaction and RNA-RNA, interaction. Here we report the first crystal structure of a bulged RNA, tetraplex at 1.1 A resolution. The hexamer r(U)(BrdG)r(UGGU) forms a, parallel tetraplex with the uridine sandwiched by guanines bulging out., The bulged uridine adopts the syn glycosidic conformation and its O2 and, N3 atoms face outwards, serving as an effective recognition and, interaction site. The bulge formation both widens the groove width and, changes the groove hydrogen-bonding pattern on its 5' side. However, the, bulge does not make any bends or kinks in the tetraplex structure. The, present study demonstrates the dramatic difference between uridine and, guanine in forming tetraplex structure. In addition, both G(syn) tetrad, and G(anti) tetrad have been observed. They display the same base-pairing, pattern and similar C1'-C1' distance but different hydrogen-bonding, patterns in the groove.

About this Structure

1P79 is a Protein complex structure of sequences from [1] with K as ligand. Full crystallographic information is available from OCA.

Reference

Crystal structure of a bulged RNA tetraplex at 1.1 a resolution: implications for a novel binding site in RNA tetraplex., Pan B, Xiong Y, Shi K, Sundaralingam M, Structure. 2003 Nov;11(11):1423-30. PMID:14604532

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