1p7c

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spinqualitylabelsall models 1p7c, resolution 2.10Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

Crystal Structure of HSV1-TK complexed with TP5A

Overview

Crystal structures of equine herpesvirus type-4 thymidine kinase (EHV4-TK), in complex with (i). thymidine and ADP, (ii). thymidine and SO(4) and the, bisubstrate analogs, (iii). TP(4)A, and (iv). TP(5)A have been solved., Additionally, the structure of herpes simplex virus type-1 thymidine, kinase (HSV1-TK) in complex with TP(5)A has been determined. These are the, first structures of nucleoside kinases revealing conformational, transitions upon binding of bisubstrate analogs. The structural basis for, the dual thymidine and thymidylate kinase activity of these TKs is, elucidated. While the active sites of HSV1-TK and EHV4-TK resemble one, another, notable differences are observed in the Lid regions and in the, way the enzymes bind the base of the phosphoryl-acceptor. The latter, difference could partly explain the higher activity of EHV4-TK toward the, prodrug ganciclovir.

About this Structure

1P7C is a Single protein structure of sequence from Human herpesvirus 4 with SO4, THM and T5A as ligands. Active as Thymidine kinase, with EC number 2.7.1.21 Full crystallographic information is available from OCA.

Reference

Structural basis for the dual thymidine and thymidylate kinase activity of herpes thymidine kinases., Gardberg A, Shuvalova L, Monnerjahn C, Konrad M, Lavie A, Structure. 2003 Oct;11(10):1265-77. PMID:14527394

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