1pk4

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spinqualitylabelsall models 1pk4, resolution 1.9Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

CRYSTAL AND MOLECULAR STRUCTURE OF HUMAN PLASMINOGEN KRINGLE 4 REFINED AT 1.9-ANGSTROMS RESOLUTION

Contents 1 Overview 2 Disease 3 About this Structure 4 Reference

Overview

The crystal structure of human plasminogen kringle 4 (PGK4) has been, solved by molecular replacement using the bovine prothrombin kringle 1, (PTK1) structure as a model and refined by restrained least-squares, methods to an R factor of 14.2% at 1.9-A resolution. The K4 structure is, similar to that of PTK1, and an insertion of one residue at position 59 of, the latter has minimal effect on the protein folding. The PGK4 structure, is highly stabilized by an internal hydrophobic core and an extensive, hydrogen-bonding network. Features new to this kringle include a cis, peptide bond at Pro30 and the presence of two alternate, perpendicular, and equally occupied orientations for the Cys75 side chain. The K4, lysine-binding site consists of a hydrophobic trough formed by the Trp62, and Trp72 indole rings, with anionic (Asp55/Asp57) and cationic, (Lys35/Arg71) charge pairs at either end. With the adjacent Asp5 and Arg32, residues, these result in triply charged anionic and cationic clusters (pH, of crystals at 6.0), which, in addition to the unusually high, accessibility of the Trp72 side chain, serve as an obvious marker of the, binding site on the K4 surface. A complex intermolecular interaction, occurs between the binding sites of symmetry-related molecules involving a, highly ordered sulfate anion of solvation in which the Arg32 side chain of, a neighboring kringle occupies the binding site.

Disease

Known diseases associated with this structure: Conjunctivitis, ligneous OMIM:[173350], Plasminogen Tochigi disease OMIM:[173350], Plasminogen deficiency, types I and II OMIM:[173350], Thrombophilia, dysplasminogenemic OMIM:[173350]

About this Structure

1PK4 is a Single protein structure of sequence from Homo sapiens with as ligand. Full crystallographic information is available from OCA.

Reference

Crystal and molecular structure of human plasminogen kringle 4 refined at 1.9-A resolution., Mulichak AM, Tulinsky A, Ravichandran KG, Biochemistry. 1991 Oct 29;30(43):10576-88. PMID:1657148

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