1q1j
From Proteopedia
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Crystal Structure Analysis of anti-HIV-1 Fab 447-52D in complex with V3 peptide
Overview
447-52D is a human monoclonal antibody isolated from a heterohybridoma, derived from an HIV-1-infected individual. This antibody recognizes the, hypervariable gp120 V3 loop, and neutralizes both X4 and R5 primary, isolates, making it one of the most effective anti-V3 antibodies, characterized to date. The crystal structure of the 447-52D Fab in complex, with a 16-mer V3 peptide at 2.5 A resolution reveals that the peptide beta, hairpin forms a three-stranded mixed beta sheet with complementarity, determining region (CDR) H3, with most of the V3 side chains exposed to, solvent. Sequence specificity is conferred through interaction of the, type-II turn (residues GPGR) at the apex of the V3 hairpin with the base, of CDR H3. This novel mode of peptide-antibody recognition enables the, antibody to bind to many different V3 sequences where only the GPxR core, epitope is absolutely required.
About this Structure
1Q1J is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Structural rationale for the broad neutralization of HIV-1 by human monoclonal antibody 447-52D., Stanfield RL, Gorny MK, Williams C, Zolla-Pazner S, Wilson IA, Structure. 2004 Feb;12(2):193-204. PMID:14962380
Page seeded by OCA on Thu Nov 8 14:23:33 2007
