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DNA DECAMER DUPLEX CONTAINING T5-T6 PHOTOADDUCT

Overview

The cis - syn dimer is the major DNA photoproduct produced by UV, irradiation. In order to determine the origin of the mutagenic property of, the cis - syn dimer, we used NMR restraints and molecular dynamics to, determine the solution structure of a DNA decamer duplex containing a, wobble pair between the 3'-T of the cis - syn dimer and the opposite T, residue (CS/TA duplex). The solution structure of the CS/TA duplex, revealed that the 3'-T x T base pair of the cis - syn dimer had base pair, geometry that was significantly different from the canonical Watson-Crick, base pair and caused destabilization and conformational distortion of its, 3'-region. However, a 3'-T x A base pair at the cis - syn dimer within, this related DNA decamer maintains the normal Watson-Crick base pair, geometry and causes little distortion in the conformation of its 3'-side., Our results show that in spite of its stable hydrogen bonding, the, insertion of a T residue opposite the 3'-T of the cis - syn dimer is, inhibited by structural distortion caused by the 3'-T x T base pair. This, may explain why the frequency of the 3'-T-->A transversion, which is the, major mutation produced by the cis - syn dimer, is only 4%.

About this Structure

1QL5 is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.

Reference

Solution structure of the DNA decamer duplex containing a 3'-T x T basepair of the cis-syn cyclobutane pyrimidine dimer: implication for the mutagenic property of the cis-syn dimer., Lee JH, Choi YJ, Choi BS, Nucleic Acids Res. 2000 Apr 15;28(8):1794-801. PMID:10734199

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