1qoi

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spinqualitylabelsall models 1qoi, resolution 2.00Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

U4/U6 SNRNP-SPECIFIC CYCLOPHILIN SNUCYP-20

Contents 1 Overview 2 Disease 3 About this Structure 4 Reference

Overview

The cyclophilin SnuCyp-20 is a specific component of the human U4/U6 small, nuclear ribonucleoprotein particle involved in the nuclear splicing of, pre-mRNA. It stably associates with the U4/U6-60kD and -90kD proteins, the, human orthologues of the Saccharomyces cerevisiae Prp4 and Prp3 splicing, factors. We have determined the crystal structure of SnuCyp-20 at 2.0-A, resolution by molecular replacement. The structure of SnuCyp-20 closely, resembles that of human cyclophilin A (hCypA). In particular, the, catalytic centers of SnuCyp-20 and hCypA superimpose perfectly, which is, reflected by the observed peptidyl-prolyl-cis/trans-isomerase activity of, SnuCyp-20. The surface properties of both proteins, however, differ, significantly. Apart from seven additional amino-terminal residues, the, insertion of five amino acids in the loop alpha1-beta3 and of one amino, acid in the loop alpha2-beta8 changes the conformations of both loops. The, enlarged loop alpha1-beta3 is involved in the formation of a wide cleft, with predominantly hydrophobic character. We propose that this enlarged, loop is required for the interaction with the U4/U6-60kD protein.

Disease

Known diseases associated with this structure: Cardiomyopathy, dilated OMIM:[605906], Cardiomyopathy, dilated, with left ventricular noncompaction OMIM:[605906], Myopathy, myofibrillar, ZASP-related OMIM:[605906]

About this Structure

1QOI is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Crystal structure of the human U4/U6 small nuclear ribonucleoprotein particle-specific SnuCyp-20, a nuclear cyclophilin., Reidt U, Reuter K, Achsel T, Ingelfinger D, Luhrmann R, Ficner R, J Biol Chem. 2000 Mar 17;275(11):7439-42. PMID:10713041

Page seeded by OCA on Mon Nov 12 18:55:10 2007