1qtw
From Proteopedia
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HIGH-RESOLUTION CRYSTAL STRUCTURE OF THE ESCHERICHIA COLI DNA REPAIR ENZYME ENDONUCLEASE IV
Overview
Endonuclease IV is the archetype for a conserved apurinic/apyrimidinic, (AP) endonuclease family that primes DNA repair synthesis by cleaving the, DNA backbone 5' of AP sites. The crystal structures of Endonuclease IV and, its AP-DNA complex at 1.02 and 1.55 A resolution reveal how an alpha8beta8, TIM barrel fold can bind dsDNA. Enzyme loops intercalate side chains at, the abasic site, compress the DNA backbone, bend the DNA approximately 90, degrees, and promote double-nucleotide flipping to sequester the, extrahelical AP site in an enzyme pocket that excludes undamaged, nucleotides. These structures suggest three Zn2+ ions directly participate, in phosphodiester bond cleavage and prompt hypotheses that, double-nucleotide flipping and sharp bending by AP endonucleases provide, exquisite damage specificity while aiding subsequent base excision repair, pathway progression.
About this Structure
1QTW is a Single protein structure of sequence from Escherichia coli with ZN as ligand. Active as Deoxyribonuclease IV (phage-T(4)-induced), with EC number 3.1.21.2 Full crystallographic information is available from OCA.
Reference
Structure of the DNA repair enzyme endonuclease IV and its DNA complex: double-nucleotide flipping at abasic sites and three-metal-ion catalysis., Hosfield DJ, Guan Y, Haas BJ, Cunningham RP, Tainer JA, Cell. 1999 Aug 6;98(3):397-408. PMID:10458614
Page seeded by OCA on Wed Nov 21 00:58:56 2007
