1qv8
From Proteopedia
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B-DNA Dodecamer d(CGCGAATTCGCG)2 complexed with proamine
Overview
New analogues of the minor groove binding ligand Hoechst 33342 have been, investigated in an attempt to improve radioprotective activity. The, synthesis, DNA binding, and in vitro radioprotective properties of, methylproamine, the most potent derivative, are reported. Experiments with, V79 cells have shown that methylproamine is approximately 100-fold more, potent than the classical aminothiol radioprotector WR1065. The crystal, structures of methylproamine and proamine complexes with the dodecamer, d(CGCGAATTCGCG)(2) confirm that the new analogues also are minor groove, binders. It is proposed that the DNA-bound methylproamine ligand acts as a, reducing agent by an electron transfer mechanism, repairing transient, radiation-induced oxidizing species on DNA.
About this Structure
1QV8 is a Protein complex structure of sequences from [1] with BBZ as ligand. Full crystallographic information is available from OCA.
Reference
In vitro studies with methylproamine: a potent new radioprotector., Martin RF, Broadhurst S, Reum ME, Squire CJ, Clark GR, Lobachevsky PN, White JM, Clark C, Sy D, Spotheim-Maurizot M, Kelly DP, Cancer Res. 2004 Feb 1;64(3):1067-70. PMID:14871839
Page seeded by OCA on Sun Nov 25 04:27:28 2007
