1rde
From Proteopedia
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NMR structure of the thrombin-binding DNA aptamer stabilized by Sr2+
Overview
The structure of thrombin-binding DNA aptamer complexed with a single Sr2+, ion (Sr2+:TBA complex) has been determined using NMR spectroscopy and, restrained molecular dynamics simulations. The quadruplex structure for, the Sr2+:TBA complex is similar in topology, but distinct in structure, from that previously reported for the K+:TBA complex. The inter-tetrad, distance of the Sr2+:TBA complex is 3.8 angstroms, or 0.7 angstroms larger, than in the K+:TBA complex. This substantial difference can be attributed, to a different binding site for Sr2+ in the Sr2+:TBA complex than for K+, in the K+:TBA complex. The Sr2+:TBA complex assumes a 1:1 stoichiometry, and it is very likely that the Sr2+ ion simultaneously interacts with the, eight O6 atoms of the two G-tetrads. The results indicate that quadruplex, DNA structures are highly sensitive to the presence of specific metal, ions. The binding of specific metal ions may modulate the biological, activity of quadruplex DNA structures in vivo.
About this Structure
1RDE is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
NMR structure of the thrombin-binding DNA aptamer stabilized by Sr2+., Mao X, Marky LA, Gmeiner WH, J Biomol Struct Dyn. 2004 Aug;22(1):25-33. PMID:15214802
Page seeded by OCA on Sat Nov 24 22:32:15 2007
