1rou
From Proteopedia
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STRUCTURE OF FKBP59-I, THE N-TERMINAL DOMAIN OF A 59 KDA FK506-BINDING PROTEIN, NMR, 22 STRUCTURES
Overview
FKBP59 is a protein usually associated with heat-shock protein hsp90 and, steroid receptors. The N-terminal domain of the rabbit liver protein (149, amino acids) has a sequence homology with FKBP12, binds FK506, immunosuppressor, and has a peptidyl-prolyl cis-trans isomerase activity., The three-dimensional structure of this domain (FKBP59-I) was determined, using homo- and heteronuclear multidimensional NMR spectroscopy, distance, geometry, and molecular dynamics methods. Structure calculations used 1290, interproton distance restraints derived from nuclear Overhauser, enhancement measurements, 29 dihedral phi angle restraints, and 92, hydrogen bond restraints. For the final 22 structures, the root mean, square distance from the mean atomic coordinates, calculated for, well-defined secondary structure fragments, is 0.47 +/- 0.05 and 1.26 +/-, 0.15 A for backbone heavy atoms (N, C alpha, C') and for all non-hydrogen, atoms, respectively. The global fold contains a twisted six-stranded, antiparallel beta-sheet and a short alpha-helix packed on the hydrophobic, side of the sheet. The 20 N-terminal and 12 C-terminal amino acids of the, domain are disordered. The main-chain structure of FKBP59-I is globally, similar to the NMR-derived and X-ray structures of unbound FKBP12. An, unusual hydrogen bond interaction between the indole amino proton of Trp, 89 and the aromatic cycle of Phe 129 was observed. This gives a large, upfield shift (-4.8 ppm) and a significant exchange protection factor. The, implications of the present structure determination on the ligand binding, of FKBP59 are discussed.
About this Structure
1ROU is a Single protein structure of sequence from Oryctolagus cuniculus. Full crystallographic information is available from OCA.
Reference
Three-dimensional structure of the immunophilin-like domain of FKBP59 in solution., Craescu CT, Rouviere N, Popescu A, Cerpolini E, Lebeau MC, Baulieu EE, Mispelter J, Biochemistry. 1996 Aug 27;35(34):11045-52. PMID:8780506
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