1ry4
From Proteopedia
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NMR Structure of the CRIB-PDZ module of Par-6
Overview
Regulation of protein interaction domains is required for cellular, signaling dynamics. Here, we show that the PDZ protein interaction domain, from the cell polarity protein Par-6 is regulated by the Rho GTPase Cdc42., Cdc42 binds to a CRIB domain adjacent to the PDZ domain, increasing the, affinity of the Par-6 PDZ for its carboxy-terminal ligand by approximately, 13-fold. Par-6 PDZ regulation is required for function as mutational, disruption of Cdc42-Par-6 PDZ coupling leads to inactivation of Par-6 in, polarized MDCK epithelial cells. Structural analysis reveals that the free, PDZ domain has several deviations from the canonical PDZ conformation that, account for its low ligand affinity. Regulation results from a, Cdc42-induced conformational transition in the CRIB-PDZ module that causes, the PDZ to assume a canonical, high-affinity PDZ conformation. The coupled, CRIB and PDZ architecture of Par-6 reveals how simple binding domains can, be combined to yield complex regulation.
About this Structure
1RY4 is a Single protein structure of sequence from Drosophila melanogaster. Full crystallographic information is available from OCA.
Reference
Cdc42 regulates the Par-6 PDZ domain through an allosteric CRIB-PDZ transition., Peterson FC, Penkert RR, Volkman BF, Prehoda KE, Mol Cell. 2004 Mar 12;13(5):665-76. PMID:15023337
Page seeded by OCA on Sat Nov 24 23:29:53 2007
