1s2f
From Proteopedia
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Average solution structure of a pseudo-5'-splice site from the negative regulator of splicing of Rous Sarcoma virus
Overview
Control of Rous sarcoma virus RNA splicing depends in part on the, interaction of U1 and U11 snRNPs with an intronic RNA element called the, negative regulator of splicing (NRS). A 23mer RNA hairpin (NRS23) of the, NRS directly binds U1 and U11 snRNPs. Mutations that disrupt base-pairing, between the loop of NRS23 and U1 snRNA abolish its negative control of, splicing. We have determined the solution structure of NRS23 using NOEs, torsion angles, and residual dipolar couplings that were extracted from, multidimensional heteronuclear NMR spectra. Our structure showed that the, 6-bp stem of NRS23 adopts a nearly A-form duplex conformation. The loop, which consists of 11 residues according to secondary structure probing, was in a closed conformation. U913, the first residue in the loop, was, bulged out or dynamic, and loop residues G914-C923, G915-U922, and, U916-A921 were base-paired. The remaining UUGU tetraloop sequence did not, adopt a stable structure and appears flexible in solution. This tetraloop, differs from the well-known classes of tetraloops (GNRA, CUYG, UNCG) in, terms of its stability, structure, and function. Deletion of the bulged, U913, which is not complementary to U1 snRNA, increased the melting, temperature of the RNA hairpin. This hyperstable hairpin exhibited a, significant decrease in binding to U1 snRNP. Thus, the structure of the, NRS RNA, as well as its sequence, is important for interaction with U1, snRNP and for splicing suppression.
About this Structure
1S2F is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
Solution structure of the pseudo-5' splice site of a retroviral splicing suppressor., Cabello-Villegas J, Giles KE, Soto AM, Yu P, Mougin A, Beemon KL, Wang YX, RNA. 2004 Sep;10(9):1388-98. PMID:15317975
Page seeded by OCA on Sat Nov 24 23:45:14 2007
