This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
1s69
From Proteopedia
|
The X-ray structure of the cyanobacteria Synechocystis hemoglobin "cyanoglobin" with cyanide ligand
Overview
The crystal structures of cyanide and azide-bound forms of the truncated, hemoglobin from Synechocystis are presented at 1.8 angstroms resolution. A, comparison with the structure of the endogenously liganded protein reveals, a conformational shift unprecedented in hemoglobins, and provides the, first picture of a hexacoordinate hemoglobin in both the bis-histidyl and, the exogenously coordinated states. The structural changes between the, different conformations are confined to two regions of the protein; the B, helix, and the E helix, including the EF loop. A molecular "hinge", controlling movement of the E helix is observed in the EF loop, which is, composed of three principal structural elements: Arg64, the, heme-d-propionate, and a three-residue extension of the F helix., Additional features of the structural transition between the two protein, conformations are discussed as they relate to the complex ligand-binding, behavior observed in hexacoordinate hemoglobins, and the potential, physiological function of this class of proteins.
About this Structure
1S69 is a Single protein structure of sequence from Synechocystis sp. with , and as ligands. Full crystallographic information is available from OCA.
Reference
Crystallographic analysis of synechocystis cyanoglobin reveals the structural changes accompanying ligand binding in a hexacoordinate hemoglobin., Trent JT 3rd, Kundu S, Hoy JA, Hargrove MS, J Mol Biol. 2004 Aug 20;341(4):1097-108. PMID:15289104
Page seeded by OCA on Fri Feb 15 16:52:31 2008
Categories: Single protein | Synechocystis sp. | Hargrove, M.S. | Hoy, J.A. | III, J.T.Trent. | Kundu, S. | CYN | FLC | HEM | 2 on 2 helical fold | Cyanobacteria | Globin | Heme | Hemoglobin | Hexacoordinate | Iron | Oxygen binding | Truncated
