1siz
From Proteopedia
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Crystal structure of the [Fe3S4]-ferredoxin from the hyperthermophilic archaeon Pyrococcus furiosus
Overview
The structure of [Fe(3)S(4)]-ferredoxin from the hyperthermophilic, archaeon Pyrococcus furiosus has been determined to 1.5 A resolution from, a crystal belonging to space group P2(1) with two molecules in the, asymmetric unit. The structure has been solved with molecular replacement, by use of the ferredoxin from Thermotoga maritima. The fold is similar to, that of related monocluster ferredoxins and contains two double-stranded, antiparallel beta-sheets and two alpha-helices. The hydrophobic, interaction between Trp2 and Tyr46 is confirmed, linking the C-terminus to, the longer alpha-helix. The structure contains a double-conformation, disulfide bond existing in a left-handed and a right-handed spiral, conformation. The crystal packing reveals a beta-sheet interaction, which, supports the suggestion that P. furiosus ferredoxin is a functional dimer., The extraordinary thermostability of P. furiosus ferredoxin is further, discussed.
About this Structure
1SIZ is a Single protein structure of sequence from Pyrococcus furiosus with 3CO and F3S as ligands. Full crystallographic information is available from OCA.
Reference
The 1.5 A resolution crystal structure of [Fe3S4]-ferredoxin from the hyperthermophilic archaeon Pyrococcus furiosus., Nielsen MS, Harris P, Ooi BL, Christensen HE, Biochemistry. 2004 May 11;43(18):5188-94. PMID:15122884
Page seeded by OCA on Wed Nov 21 02:24:50 2007
