1sjg
From Proteopedia
|
Solution Structure of T4moC, the Rieske Ferredoxin Component of the Toluene 4-Monooxygenase Complex
Overview
Toluene 4-monooxygenase, a four-protein complex from Pseudomonas mendocina, KR1, catalyzes the NADH- and O(2)-dependent hydroxylation of toluene to, form p-cresol. The solution structure of the 112-amino-acid Rieske, ferredoxin component, T4moC, was determined from 2D and 3D (1)H, (13)C, and (15)N NMR data. The structural model was refined through simulated, annealing by molecular dynamics in torsion angle space with input from, 1650 experimental restraints, including 1264 inter-proton distance, restraints obtained from NOEs, 247 non-redundant intra-residue NOEs, 26, hydrogen bond restraints, and 113 dihedral angle ( phi, psi) restraints., The 20 calculated conformers that best satisfied the input restraints were, submitted to refinement in explicit solvent to improve the stereochemical, quality. With exclusion of ill-defined N- and C-terminal segments (Ser2;, His111-Ser112) and residues near to the [2Fe-2S] cluster, the atomic root, mean square deviation for the 20 conformers with respect to the mean, coordinates was 1.09 A for the backbone and 1.60 A for all non-hydrogen, atoms. The T4moC structure consists of 10 beta-strands arranged in the, three anti-parallel beta-sheet topology observed in all Rieske [2Fe-2S], domain proteins. The S(gamma) of Cys45 and Cys64 and the N(delta1) of, His47 and His67 provide the ligands to the [2Fe-2S] cluster of T4moC., (1)H-(15)N HSQC measurements show that both His47-N(epsilon2) and, His67-N(epsilon2) are protonated at the pH of the NMR experiments., Comparisons are made between the present NMR structure, previous, paramagnetic NMR studies of T4moC, and the X-ray structures of other, members of the Rieske protein family.
About this Structure
1SJG is a Single protein structure of sequence from Pseudomonas mendocina with FES as ligand. Full crystallographic information is available from OCA.
Reference
Solution structure of T4moC, the Rieske ferredoxin component of the toluene 4-monooxygenase complex., Skjeldal L, Peterson FC, Doreleijers JF, Moe LA, Pikus JD, Westler WM, Markley JL, Volkman BF, Fox BG, J Biol Inorg Chem. 2004 Dec;9(8):945-53. Epub 2004 Sep 25. PMID:15452777[[Category: rieske [2fe-2s] domain]]
Page seeded by OCA on Wed Nov 21 02:25:17 2007
