1soo
From Proteopedia
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ADENYLOSUCCINATE SYNTHETASE INHIBITED BY HYDANTOCIDIN 5'-MONOPHOSPHATE
Overview
(+)-Hydantocidin, a recently discovered natural spironucleoside with, potent herbicidal activity, is shown to be a proherbicide that, after, phosphorylation at the 5' position, inhibits adenylosuccinate synthetase, an enzyme involved in de novo purine synthesis. The mode of binding of, hydantocidin 5'-monophosphate to the target enzyme was analyzed by, determining the crystal structure of the enzyme-inhibitor complex at 2.6-A, resolution. It was found that adenylosuccinate synthetase binds the, phosphorylated compound in the same fashion as it does adenosine, 5'-monophosphate, the natural feedback regulator of this enzyme. This work, provides the first crystal structure of a herbicide-target complex, reported to date.
About this Structure
1SOO is a Single protein structure of sequence from Escherichia coli with SO4, NA, H5P and BME as ligands. Active as Adenylosuccinate synthase, with EC number 6.3.4.4 Full crystallographic information is available from OCA.
Reference
The mode of action and the structure of a herbicide in complex with its target: binding of activated hydantocidin to the feedback regulation site of adenylosuccinate synthetase., Fonne-Pfister R, Chemla P, Ward E, Girardet M, Kreuz KE, Honzatko RB, Fromm HJ, Schar HP, Grutter MG, Cowan-Jacob SW, Proc Natl Acad Sci U S A. 1996 Sep 3;93(18):9431-6. PMID:8790347
Page seeded by OCA on Wed Nov 21 02:31:48 2007
