1stc
From Proteopedia
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CAMP-DEPENDENT PROTEIN KINASE, ALPHA-CATALYTIC SUBUNIT IN COMPLEX WITH STAUROSPORINE
Overview
BACKGROUND: Staurosporine inhibits most protein kinases at low nanomolar, concentrations. As most tyrosine kinases, along with many serine/threonine, kinases, are either proto oncoproteins or are involved in oncogenic, signaling, the development of protein kinase inhibitors is a primary goal, of cancer research. Staurosporine and many of its derivatives have, significant biological effects, and are being tested as anticancer drugs., To understand in atomic detail the mode of inhibition and the parameters, of high-affinity binding of staurosporine to protein kinases, the molecule, was cocrystallized with the catalytic subunit of cAMP-dependent protein, kinase. RESULTS: The crystal structure of the protein kinase catalytic, subunit with staurosporine bound to the adenosine pocket shows, considerable induced-fit rearrangement of the enzyme and a unique open, conformation. The inhibitor mimics several aspects of adenosine binding, including both polar and nonpolar interactions with enzyme residues, and, induces conformational changes of neighboring enzyme residues., CONCLUSIONS: The results explain the high inhibitory potency of, staurosporine, and also illustrate the flexibility of the protein kinase, active site. The structure, therefore, is not only useful for the design, of improved anticancer therapeutics and signaling drugs, but also provides, a deeper understanding of the conformational flexibility of the protein, kinase.
About this Structure
1STC is a Protein complex structure of sequences from Bos taurus with STU as ligand. Active as Non-specific serine/threonine protein kinase, with EC number 2.7.11.1 Full crystallographic information is available from OCA.
Reference
Staurosporine-induced conformational changes of cAMP-dependent protein kinase catalytic subunit explain inhibitory potential., Prade L, Engh RA, Girod A, Kinzel V, Huber R, Bossemeyer D, Structure. 1997 Dec 15;5(12):1627-37. PMID:9438863
Page seeded by OCA on Wed Nov 21 02:38:56 2007
