1t1m

From Proteopedia

Revision as of 23:22, 24 November 2007 by OCA (Talk | contribs)
(diff) ←Older revision | Current revision (diff) | Newer revision→ (diff)
Jump to: navigation, search

1t1m

Drag the structure with the mouse to rotate

Binding position of ribosome recycling factor (RRF) on the E. coli 70S ribosome

Overview

After the termination step of protein synthesis, a deacylated tRNA and, mRNA remain associated with the ribosome. The ribosome-recycling factor, (RRF), together with elongation factor G (EF-G), disassembles this, posttermination complex into mRNA, tRNA, and the ribosome. We have, obtained a three-dimensional cryo-electron microscopic map of a complex of, the Escherichia coli 70S ribosome and RRF. We find that RRF interacts, mainly with the segments of the large ribosomal subunit's (50S) rRNA, helices that are involved in the formation of two central intersubunit, bridges, B2a and B3. The binding of RRF induces considerable, conformational changes in some of the functional domains of the ribosome., As compared to its binding position derived previously by hydroxyl radical, probing study, we find that RRF binds further inside the intersubunit, space of the ribosome such that the tip of its domain I is shifted (by, approximately 13 A) toward protein L5 within the central protuberance of, the 50S subunit, and domain II is oriented more toward the small ribosomal, subunit (30S). Overlapping binding sites of RRF, EF-G, and the P-site tRNA, suggest that the binding of EF-G would trigger the removal of deacylated, tRNA from the P site by moving RRF toward the ribosomal E site, and, subsequent removal of mRNA may be induced by a shift in the position of, 16S rRNA helix 44, which harbors part of the mRNA.

About this Structure

1T1M is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Visualization of ribosome-recycling factor on the Escherichia coli 70S ribosome: functional implications., Agrawal RK, Sharma MR, Kiel MC, Hirokawa G, Booth TM, Spahn CM, Grassucci RA, Kaji A, Frank J, Proc Natl Acad Sci U S A. 2004 Jun 15;101(24):8900-5. Epub 2004 Jun 3. PMID:15178758

Page seeded by OCA on Sun Nov 25 01:29:36 2007

Proteopedia Page Contributors and Editors (what is this?)

OCA

Personal tools