1t50
From Proteopedia
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NMR SOLUTION STRUCTURE OF APLYSIA ATTRACTIN
Overview
Water-borne protein pheromones are essential for coordination of, reproductive activities in many marine organisms. In this paper, we, describe the first structure of a pheromone protein from a marine, organism, that of attractin (58 residues) from Aplysia californica. The, NMR solution structure was determined from TOCSY, NOESY, and DQF-COSY, measurements of recombinant attractin expressed in insect cells. The, sequential resonance assignments were done with standard manual, procedures. Approximately 90% of the 949 unambiguous NOESY cross-peaks, were assigned automatically with simultaneous three-dimensional structure, calculation using our NOAH/DIAMOD/FANTOM program suite. The final bundle, of energy-refined structures is well-defined, with an average rmsd value, to the mean structure of 0.72 +/- 0.12 A for backbone and 1.32 +/- 0.11 A, for heavy atoms for amino acids 3-47. Attractin contains two antiparallel, helices, made up of residues Ile9-Gln16 and I30-S36. The NMR distance, constraints are consistent with the three disulfide bonds determined by, mass spectroscopy (C4-C41, C13-C33, and C20-C26), where the first two, could be directly determined from NOESY cross-peaks between CH beta, protons of the corresponding cysteines. The second helix contains the, (L/I)(29)IEECKTS(36) sequence conserved in attractins from five species of, Aplysia that could interact with the receptor. The sequence and structure, of this region are similar to those of the recognition helix of the Er-11, pheromone of the unicellular ciliate Euplotes raikovi, suggesting a, possible common pathway for intercellular communication of these two, distinct pheromone families.
About this Structure
1T50 is a Single protein structure of sequence from Aplysia californica. This structure superseeds the now removed PDB entry 1NJ7. Full crystallographic information is available from OCA.
Reference
NMR solution structure of attractin, a water-borne protein pheromone from the mollusk Aplysia californica., Garimella R, Xu Y, Schein CH, Rajarathnam K, Nagle GT, Painter SD, Braun W, Biochemistry. 2003 Aug 26;42(33):9970-9. PMID:12924946
Page seeded by OCA on Wed Nov 21 02:59:52 2007
