1tgr

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spinqualitylabelsall models 1tgr, resolution 1.42Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

Crystal Structure of mini-IGF-1(2)

Contents 1 Overview 2 Disease 3 About this Structure 4 Reference

Overview

Insulin and insulin-like growth factor 1 (IGF-1) share a homologous, sequence, a similar three-dimensional structure and weakly overlapping, biological activity, but IGF-1 folds into two thermodynamically stable, disulfide isomers, while insulin folds into one unique stable tertiary, structure. This is a very interesting phenomenon in which one amino acid, sequence encodes two three-dimensional structures, and its molecular, mechanism has remained unclear for a long time. In this study, the crystal, structure of mini-IGF-1(2), a disulfide isomer of an artificial analog of, IGF-1, was solved by the SAD/SIRAS method using our in-house X-ray source., Evidence was found in the structure showing that the intra-A-chain/domain, disulfide bond of some molecules was broken; thus, it was proposed that, disulfide isomerization begins with the breakdown of this disulfide bond., Furthermore, based on the structural comparison of IGF-1 and insulin, a, new assumption was made that in insulin the several hydrogen bonds formed, between the N-terminal region of the B-chain and the intra-A-chain, disulfide region of the A-chain are the main reason for the stability of, the intra-A-chain disulfide bond and for the prevention of disulfide, isomerization, while Phe B1 and His B5 are very important for the, formation of these hydrogen bonds. Moreover, the receptor binding property, of IGF-1 was analyzed in detail based on the structural comparison of, mini-IGF-1(2), native IGF-1, and small mini-IGF-1.

Disease

Known disease associated with this structure: Growth retardation with deafness and mental retardation due to IGF1 deficiency OMIM:[147440]

About this Structure

1TGR is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

1.42A crystal structure of mini-IGF-1(2): an analysis of the disulfide isomerization property and receptor binding property of IGF-1 based on the three-dimensional structure., Yun CH, Tang YH, Feng YM, An XM, Chang WR, Liang DC, Biochem Biophys Res Commun. 2005 Jan 7;326(1):52-9. PMID:15567151

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