1tjy
From Proteopedia
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Crystal Structure of Salmonella typhimurium AI-2 receptor LsrB in complex with R-THMF
Overview
Bacterial populations use cell-cell communication to coordinate, community-wide regulation of processes such as biofilm formation, virulence, and bioluminescence. This phenomenon, termed quorum sensing, is, mediated by small molecule signals known as autoinducers. While most, autoinducers are species specific, autoinducer-2 (AI-2), first identified, in the marine bacterium Vibrio harveyi, is produced and detected by many, Gram-negative and Gram-positive bacteria. The crystal structure of the V., harveyi AI-2 signaling molecule bound to its receptor protein revealed an, unusual furanosyl borate diester. Here, we present the crystal structure, of a second AI-2 signal binding protein, LsrB from Salmonella typhimurium., We find that LsrB binds a chemically distinct form of the AI-2 signal, (2R,4S)-2-methyl-2,3,3,4-tetrahydroxytetrahydrofuran (R-THMF), that lacks, boron. Our results demonstrate that two different species of bacteria, recognize two different forms of the autoinducer signal, both derived from, 4,5-dihydroxy-2,3-pentanedione (DPD), and reveal new sophistication in the, chemical lexicon used by bacteria in interspecies signaling.
About this Structure
1TJY is a Single protein structure of sequence from Salmonella typhimurium lt2 with PAV as ligand. Full crystallographic information is available from OCA.
Reference
Salmonella typhimurium recognizes a chemically distinct form of the bacterial quorum-sensing signal AI-2., Miller ST, Xavier KB, Campagna SR, Taga ME, Semmelhack MF, Bassler BL, Hughson FM, Mol Cell. 2004 Sep 10;15(5):677-87. PMID:15350213
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