1tkk
From Proteopedia
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The Structure of a Substrate-Liganded Complex of the L-Ala-D/L-Glu Epimerase from Bacillus subtilis
Overview
The members of the mechanistically diverse enolase superfamily share a, bidomain structure formed from a (beta/alpha)7beta-barrel domain [a, modified (beta/alpha)8- or TIM-barrel] and a capping domain formed from N-, and C-terminal segments of the polypeptide. The active sites are located, at the interface between the C-terminal ends of the beta-strands in the, barrel domain and two flexible loops in the capping domain. Within this, structure, the acid/base chemistry responsible for formation and, stabilization of an enediolate intermediate derived from a carboxylate, anion substrate and the processing of it to product is "hard-wired" by, functional groups at the C-terminal ends of the beta-strands in the barrel, domain; the identity of the substrate is determined in part by the, identities of residues located at the end of the eighth beta-strand in the, barrel domain and two mobile loops in the capping domain. On the basis of, the identities of the acid/base functional groups at the ends of the, beta-strands, the currently available structure-function relationships, derived from functionally characterized members are often sufficient for, "deciphering" the identity of the chemical reaction catalyzed by, sequence-divergent members discovered in genome projects. However, insufficient structural information for liganded complexes for specifying, the identity of the substrate is available. In this paper, the structure, of the complex of L-Ala-L-Glu with the L-Ala-D/L-Glu epimerase from, Bacillus subtilis is reported. As expected for the 1,1-proton transfer, reaction catalyzed by this enzyme, the alpha-carbon of the substrate is, located between Lys 162 and Lys 268 at the ends of the second and sixth, beta-strands in the barrel domain. The alpha-ammonium group of the l-Ala, moiety is hydrogen bonded to both Asp 321 and Asp 323 at the end of the, eighth beta-strand, revealing a novel strategy for substrate recognition, in the superfamily. The delta-carboxylate group of the Glu moiety is, hydrogen bonded to Arg 24 in one of the flexible loops in the capping, domain, thereby providing a structural explanation for the restricted, substrate specificity of this epimerase [Schmidt, D. M., Hubbard, B. K., and Gerlt, J. A. (2001) Biochemistry 40, 15707-15715]. These studies, provide important new information about the structural bases for substrate, specificity in the enolase superfamily.
About this Structure
1TKK is a Single protein structure of sequence from Bacillus subtilis with MG and ALA as ligands. Full crystallographic information is available from OCA.
Reference
Evolution of enzymatic activities in the enolase superfamily: structure of a substrate-liganded complex of the L-Ala-D/L-Glu epimerase from Bacillus subtilis., Klenchin VA, Schmidt DM, Gerlt JA, Rayment I, Biochemistry. 2004 Aug 17;43(32):10370-8. PMID:15301535
Page seeded by OCA on Sun Nov 25 02:20:39 2007
