1tpl
From Proteopedia
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THE THREE-DIMENSIONAL STRUCTURE OF TYROSINE PHENOL-LYASE
Overview
Tyrosine phenol-lyase (EC 4.1.99.2) from Citrobacter freundii has been, cloned and the primary sequence deduced from the DNA sequence. From the, BrCN digest of the NaBH4-reduced holoenzyme, five peptides were purified, and sequenced. The amino acid sequences of the peptides agreed with the, corresponding parts of the tyrosine phenol-lyase sequence obtained from, the gene structure. K257 is the pyridoxal 5'-phosphate binding residue., Assisted by the sequence data, the crystal structure of apotyrosine, phenol-lyase, a pyridoxal 5'-phosphate-dependent enzyme, has been refined, to an R-factor of 16.2% at 2.3-A resolution using synchrotron radiation, diffraction data. The tetrameric molecule has 222 symmetry, with one of, the axes coincident with the crystallographic 2-fold symmetry axis of the, crystal which belongs to the space group P2(1)2(1)2 with a = 76.0 A, b =, 138.3 A, and c = 93.5 A. Each subunit comprises 14 alpha-helices and 16, beta-strands, which fold into a small and a large domain. The, coenzyme-binding lysine residue is located at the interface between the, large and small domains of one subunit and the large domain of a, crystallographically related subunit. The fold of the large, pyridoxal, 5'-phosphate binding domain and the location of the active site are, similar to that found in aminotransferases. Most of the residues which, participate in binding of pyridoxal 5'-phosphate in aminotransferases are, conserved in the structure of tyrosine phenol-lyase. Two dimers of, tyrosine phenol-lyase, each of which has a domain architecture similar to, that found in aspartate aminotransferases, are bound together through a, hydrophobic cluster in the center of the molecule and intertwined, N-terminal arms.
About this Structure
1TPL is a Single protein structure of sequence from [1] with SO4 as ligand. Active as Tyrosine phenol-lyase, with EC number 4.1.99.2 Full crystallographic information is available from OCA.
Reference
Three-dimensional structure of tyrosine phenol-lyase., Antson AA, Demidkina TV, Gollnick P, Dauter Z, von Tersch RL, Long J, Berezhnoy SN, Phillips RS, Harutyunyan EH, Wilson KS, Biochemistry. 1993 Apr 27;32(16):4195-206. PMID:7916622
Page seeded by OCA on Wed Nov 21 03:29:21 2007
