1ue3

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spinqualitylabelsall models 1ue3, resolution 2.15Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

Crystal structure of d(GCGAAAGC) containing hexaamminecobalt

Overview

A DNA fragment d(GCGAAAGC), postulated to adopt a stable mini-hairpin, structure on the basis of its extraordinary properties, has been X-ray, analyzed. Two octamers related by a crystallographic twofold symmetry are, aligned in an antiparallel fashion and associate to form a duplex, which, is maintained by two Watson-Crick G.C base pairs and a subsequent sheared, G.A pair at both ends. The central two A residues are free from base-pair, formation. The corresponding base moieties of the two strands are, intercalated and stacked on each other, forming a long column of, G(1)-C(2)-G(3)-A(4)-A(5)(*)-A(5)-A(4)(*)-G(3)(*)-C(2)(*)-G(1)(*), (asterisks indicate the counter-strand). The Watson-Crick and major-groove, sites of the four stacked adenine bases are exposed to the solvent region, suggesting a functional role. Since this structural motif is similar to, those found in the nonamers d(G(Br)CGAAAGCT) and d(G(I)CGAAAGCT), the, base-intercalated duplex may be a stable form of the specific sequence., Electrophoresis results suggest that the octamer has two states, monomeric, and dimeric, in solution depending on the Mg(2+) concentration. The, present duplex is preferred under the crystallization conditions, which, correspond to physiologically allowed conditions.

About this Structure

1UE3 is a Protein complex structure of sequences from [1] with CL, MG and NCO as ligands. Full crystallographic information is available from OCA.

Reference

Structure of d(GCGAAAGC) (hexagonal form): a base-intercalated duplex as a stable structure., Sunami T, Kondo J, Hirao I, Watanabe K, Miura KI, Takenaka A, Acta Crystallogr D Biol Crystallogr. 2004 Jan;60(Pt 1):90-6. Epub 2003 Dec, 18. PMID:14684897

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