1ulh

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spinqualitylabelsall models 1ulh, resolution 2.31Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

A short peptide insertion crucial for angiostatic activity of human tryptophanyl-tRNA synthetase

Contents 1 Overview 2 Disease 3 About this Structure 4 Reference

Overview

Human tryptophanyl-tRNA synthetase (TrpRS) is secreted into the, extracellular region of vascular endothelial cells. The splice variant, form (mini TrpRS) functions in vascular endothelial cell apoptosis as an, angiostatic cytokine. In contrast, the closely related human tyrosyl-tRNA, synthetase (TyrRS) functions as an angiogenic cytokine in its truncated, form (mini TyrRS). Here, we determined the crystal structure of human mini, TrpRS at a resolution of 2.3 A and compared the structure with those of, prokaryotic TrpRS and human mini TyrRS. Deletion of the tRNA, anticodon-binding (TAB) domain insertion, consisting of eight residues in, the human TrpRS, abolished the enzyme's apoptotic activity for endothelial, cells, whereas its translational catalysis and cell-binding activities, remained unchanged. Thus, we have identified the inserted peptide motif, that activates the angiostatic signaling.

Disease

Known disease associated with this structure: Wolcott-Rallison syndrome OMIM:[604032]

About this Structure

1ULH is a Single protein structure of sequence from Homo sapiens. Active as Tryptophan--tRNA ligase, with EC number 6.1.1.2 Full crystallographic information is available from OCA.

Reference

A short peptide insertion crucial for angiostatic activity of human tryptophanyl-tRNA synthetase., Kise Y, Lee SW, Park SG, Fukai S, Sengoku T, Ishii R, Yokoyama S, Kim S, Nureki O, Nat Struct Mol Biol. 2004 Feb;11(2):149-56. Epub 2004 Jan 11. PMID:14730354

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