1ux8
From Proteopedia
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X-RAY STRUCTURE OF TRUNCATED OXYGEN-AVID HAEMOGLOBIN FROM BACILLUS SUBTILIS
Overview
The group II truncated hemoglobin from Bacillus subtilis has been cloned, expressed, purified, and characterized. B. subtilis truncated hemoglobin, is a monomeric protein endowed with an unusually high oxygen affinity (in, the nanomolar range) such that the apparent thermodynamic binding constant, for O2 exceeds that for CO by 1 order of magnitude. The kinetic basis of, the high oxygen affinity resides mainly in the very slow rate of ligand, release. The extremely stable ferrous oxygenated adduct is resistant to, oxidation, which can be achieved only with oxidant in large excess, e.g., ferricyanide in 50-fold molar excess. The three-dimensional crystal, structure of the cyano-Met derivative was determined at 2.15 A resolution., Although the overall fold resembles that of other truncated hemoglobins, the distal heme pocket displays a unique array of hydrophilic side chains, in the topological positions that dominate the steric interaction with, iron-bound ligands. In fact, the Tyr-B10, Thr-E7, and Gln-E11 oxygens on, one side of the heme pocket and the Trp-G8 indole NE1 nitrogen on the, other form a novel pattern of the "ligand-inclusive hydrogen bond network", described for mycobacterial HbO. On the proximal side, the histidine, residue is in an unstrained conformation, and the iron-His bond is, unusually short (1.91 A).
About this Structure
1UX8 is a Single protein structure of sequence from Bacillus subtilis with , and as ligands. Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
The truncated oxygen-avid hemoglobin from Bacillus subtilis: X-ray structure and ligand binding properties., Giangiacomo L, Ilari A, Boffi A, Morea V, Chiancone E, J Biol Chem. 2005 Mar 11;280(10):9192-202. Epub 2004 Dec 7. PMID:15590662
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