This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
1v33
From Proteopedia
|
Crystal structure of DNA primase from Pyrococcus horikoshii
Overview
BACKGROUND: In chromosomal DNA replication, DNA primase initiates the, synthesis of a dinucleotide on a single-stranded template DNA, and, elongates it to form a primer RNA for the replicative DNA polymerase., Although the apo-structure of an archaeal primase has been reported, the, mechanism of primer synthesis by the eukaryotic-type primase still remains, to be elucidated. RESULTS: In this study, we present the crystal structure, of the eukaryotic-type DNA primase from the hyperthermophilic archaeon, (Pyrococcus horikoshii) with the uridine 5'-triphosphate (UTP). In the, present primase-UTP complex, the primase binds the triphosphate moiety of, the UTP at the active site, which includes Asp95, Asp97, and Asp280, the, essential residues for the nucleotidyl transfer reaction. CONCLUSION: The, nucleotide binding geometry in this complex explains the previous, biochemical analyses of the eukaryotic primase. Based on the complex, structure, we constructed a model between the DNA primase and a, primer/template DNA for the primer synthesis. This model facilitates the, comprehension of the reported features of DNA primase.
About this Structure
1V33 is a Single protein structure of sequence from Pyrococcus horikoshii with ZN and PO4 as ligands. Full crystallographic information is available from OCA.
Reference
Crystal structure of the Pyrococcus horikoshii DNA primase-UTP complex: implications for the mechanism of primer synthesis., Ito N, Nureki O, Shirouzu M, Yokoyama S, Hanaoka F, Genes Cells. 2003 Dec;8(12):913-23. PMID:14750947
Page seeded by OCA on Wed Nov 21 04:24:37 2007
