1v45
From Proteopedia
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Crystal Structure of human PNP complexed with 3-deoxyguanosine
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Overview
Purine nucleoside phosphorylase (PNP) is a key enzyme in the, purine-salvage pathway, which allows cells to utilize preformed bases and, nucleosides in order to synthesize nucleotides. PNP is specific for purine, nucleosides in the beta-configuration and exhibits a strong preference for, purines containing a 6-keto group and ribosyl-containing nucleosides, relative to the corresponding analogues. PNP was crystallized in complex, with ligands and data collection was performed using synchrotron, radiation. This work reports the structure of human PNP in complex with, guanosine (at 2.80 A resolution), 3'-deoxyguanosine (at 2.86 A resolution), and 8-azaguanine (at 2.85 A resolution). These structures were compared, with the PNP-guanine, PNP-inosine and PNP-immucillin-H complexes solved, previously.
Disease
Known diseases associated with this structure: Neutral lipid storage disease with myopathy OMIM:[609059], Nucleoside phosphorylase deficiency, immunodeficiency due to OMIM:[164050]
About this Structure
1V45 is a Single protein structure of sequence from Homo sapiens with SO4 and 3DG as ligands. Active as Purine-nucleoside phosphorylase, with EC number 2.4.2.1 Full crystallographic information is available from OCA.
Reference
Structure of human PNP complexed with ligands., Canduri F, Silva RG, dos Santos DM, Palma MS, Basso LA, Santos DS, de Azevedo WF Jr, Acta Crystallogr D Biol Crystallogr. 2005 Jul;61(Pt 7):856-62. Epub 2005, Jun 24. PMID:15983407
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