1vdx

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spinqualitylabelsall models 1vdx, resolution 2.40Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

Crystal Structure of a Pyrococcus horikoshii protein with similarities to 2'5' RNA-ligase

Overview

Cyclic phosphodiesterase and 2'-5' RNA ligase are members of a superfamily, of proteins which share structural similarities even though their homology, may be very low. A putative 2'-5' RNA ligase from Pyrococcus horikoshii, has been crystallized and its X-ray crystallographic structure determined, to 2.4 A. The protein crystallized in the orthorhombic space group, P2(1)2(1)2(1), with unit-cell parameters a = 44.07, b = 45.47, c = 93.17 A, and one protein monomer in the asymmetric unit. The molecular-replacement, probe was a 2'-5' RNA ligase from Thermus thermophilus which shares 30%, sequence identity. The P. horikoshii RNA ligase has some structural, features that have more in common with a cyclic phosphodiesterase from, Arabidopsis thaliana with which it has no significant homology, yet an, examination of the electrostatic surface potential clearly defines its, relationship to the T. thermophilus RNA ligase. However, the size of the, active-site cleft is smaller and less positively charged than that of the, T. thermophilus homologue, suggesting that the actual substrate may be, smaller than that previously postulated for the latter.

About this Structure

1VDX is a Single protein structure of sequence from Pyrococcus horikoshii with CL as ligand. Active as RNA ligase (ATP), with EC number 6.5.1.3 Full crystallographic information is available from OCA.

Reference

Structure of a putative 2'-5' RNA ligase from Pyrococcus horikoshii., Rehse PH, Tahirov TH, Acta Crystallogr D Biol Crystallogr. 2005 Sep;61(Pt 9):1207-12. Epub 2005, Aug 16. PMID:16131753

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